Backgroun The purpose of this study is to evaluate the hemostatic efficacy of a novel plant originated blood stopper which is called Algan Hemostatic Agent (AHA) in uncontrolled hepatectomy bleeding model. Materials and Methods The study was performed on 64 rats each of 5-7 weeks old. The rats were randomly divided into 8 groups, each consisting of 8 rats (4 groups: heparinized, 4 groups: non-heparinized). Experimental hepatectomy model was performed and saline-impregnated sponge was applied to control group for managing hemorrhage. Algan Hemostatic Agent (AHA) was applied topically in three different formulation types; AHA-impregnated gauze, gel formulation and lyophilized powder form were applied to study groups to evaluate the effect on hemorrhage control. Results There is no difference in mean weight and weight of resected liver segment among the groups. The AHA powder form was able to control the bleeding in heparinized and non-heparinized groups in 7 and 4 seconds, respectively. The AHA gel formulation was able to control the bleeding in heparinized and non-heparinized groups in 8 and 5 seconds, respectively. AHA liquid form ceased the bleeding in the heparinized and non-heparinized group in the first 15-second compress application. The bleeding time in heparinized and non-heparinized control groups in 410 and 220 seconds, respectively. The bleeding time was quite shorter in all AHA groups comparing to control group animals. Conclusion This study showed that AHA is a highly effective hemostatic agent which would be beneficial in controlling hemorrhage.
Corresponding Author: Midi A.