Objectives: The purpose of this study is to evaluate the hemostatic efficacy of a novel plant originated blood stopper which is called Algan Hemostatic Agent (AHA) in uncontrolled hepatectomy bleeding model. Methods: The study was performed on 64 rats each of 5-7 weeks old. The rats were randomly divided into 8 groups, each consisting of 8 rats (4 groups: heparinized, 4 groups: non-heparinized). Experimental hepatectomy model was performed and saline-impregnated sponge was applied to control group for managing hemorrhage. Algan Hemostatic Agent (AHA) was applied topically in three different formulation types; AHA-impregnated gauze, gel formulation and lyophilized powder form were applied to study groups to evaluate the effect on hemorrhage control. Results: There is no difference in mean weight and weight of resected liver segment among the groups. The AHA powder form was able to control the bleeding in heparinized and non-heparinized groups in 7 and 4 seconds, respectively. The AHA gel formulation was able to control the bleeding in heparinized and non-heparinized groups in 8 and 5 seconds, respectively. AHA liquid form ceased the bleeding in the heparinized and non-heparinized group in the first 15-second compress application. The bleeding time in heparinized and non-heparinized control groups in 410 and 220 seconds, respectively. The bleeding time was quite shorter in all AHA groups comparing to control group animals. Conclusion: This study showed that AHA is a highly effective hemostatic agent which would be beneficial in controlling hemorrhage. Keywords: Algan hemostaic agent, haemorrhage, liver, rat
Corresponding Author: Midi A.