Investigation Of Efficacy Of The Plant Based Algan Hemostatıc Agent, In Hepatectomy Bleedıng Model In Rats.
Ahmet Midi1, Hatice Emel Ozyurek2, Siyami Karahan3, Husamettin Ekici4, Ali Kumandas5, Irem Turkmen6, Ebranur Kocabas7, Mustafa Turk8, Ozlem Unay Demirel9, Erdem Yesilada101Bahcesehir University, Faculty Of Medicine, Pathology Department, Istanbul, 2Bahcesehir University, Faculty Of Medicine, Pediatrics Department, Section Of Hematology, Istanbul, 3Kırıkkale University Faculty Of Veterinary Medicine, Histology Department, Kırıkkale., 4Kırıkkale University Faculty Of Veterinary Medicine, Pharmacology And Toxicology Department, Kırıkkkale, 5Kırıkkale University, Veterinary Faculty, Surgery Department, Kırıkkale., 6Bahcesehir University, Faculty Of Medicine, 3rd Grade Student, Istanbul., 7Istanbul University, Faculty Of Medicine, 4th Grade Student, Istanbul., 8Kırıkkale University Faculty Of Engineering, Department Of Bioengineering, Kırıkkale., 9Bahcesehir University, Faculty Of Medicine, Biochemistry Department, Istanbul., 10Yeditepe University, Faculty Of Farmacy, Dept. Of Pharmacognosy And Phytotherapy, Ataşehir, Istanbul.
Objectives: The purpose of this study is to evaluate the hemostatic efficacy of a novel plant originated blood stopper which is called Algan Hemostatic Agent (AHA) in uncontrolled hepatectomy bleeding model. Methods: The study was performed on 64 rats each of 5-7 weeks old. The rats were randomly divided into 8 groups, each consisting of 8 rats (4 groups: heparinized, 4 groups: non-heparinized). Experimental hepatectomy model was performed and saline-impregnated sponge was applied to control group for managing hemorrhage. Algan Hemostatic Agent (AHA) was applied topically in three different formulation types; AHA-impregnated gauze, gel formulation and lyophilized powder form were applied to study groups to evaluate the effect on hemorrhage control. Results: There is no difference in mean weight and weight of resected liver segment among the groups. The AHA powder form was able to control the bleeding in heparinized and non-heparinized groups in 7 and 4 seconds, respectively. The AHA gel formulation was able to control the bleeding in heparinized and non-heparinized groups in 8 and 5 seconds, respectively. AHA liquid form ceased the bleeding in the heparinized and non-heparinized group in the first 15-second compress application. The bleeding time in heparinized and non-heparinized control groups in 410 and 220 seconds, respectively. The bleeding time was quite shorter in all AHA groups comparing to control group animals. Conclusion: This study showed that AHA is a highly effective hemostatic agent which would be beneficial in controlling hemorrhage. Keywords: Algan hemostaic agent, haemorrhage, liver, rat
Cite This Article
Midi A, Ozyurek H, Karahan S, Ekici H, Kumandas A, Turkmen I, Kocabas E, Turk M, Demirel O, Yesilada E. Investigation Of Efficacy Of The Plant Based Algan Hemostatıc Agent, In Hepatectomy Bleedıng Model In Rats.. EJMI. 2018; 2(4): 195-201
Corresponding Author: Ahmet Midi