A Snapshot of Oral Microbiota in Patients with Colorectal Cancer
Deniz Can Guven1, Koray Ergunay2, Annika Brinkmann3, Alpaslan Alp2, Fatma Nur Kittana2, Yakut Akyon2, Engin Yilmaz4, Derya Karakoc5, Erhan Hamaloglu5, Suayip Yalcin1, Andreas Nitsche3, Mutlu Hayran6, Omer Dizdar61Department of Medical Oncology, Hacettepe University Cancer Institute, Ankara, Turkey, 2Department of Medical Microbiology, Hacettepe University Faculty of Medicine, Ankara, Turkey, 3Robert Koch Institute, Centre for Biological Threats and Special Pathogens 1 (ZBS 1), Berlin, Germany, 4Department of Medical Biology, Acibadem Mehmet Ali Aydinlar University Faculty of Medicine, Istanbul, Turkey, 5Department of General Surgery, Hacettepe University Faculty of Medicine, Ankara, Turkey, 6Department of Preventive Oncology, Hacettepe University Cancer Institute, Ankara, Turkey
Objectives: Human oral microbiota is evaluated in several cancers; however, data on colorectal cancer (CRC) are very limited. Based on this, we aimed to investigate the composition of oral microbiota in CRC and to compare them with healthy controls. Methods: Saliva samples from 32 patients who were diagnosed with CRC and from 32 healthy controls in the same age group were evaluated in this study. Bacterial populations were identified through the multiplication of partial 16S rDNA sequences and comparison with databases after next-generation sequencing (Illumina MiSeq). Results: In family-level examinations, the relative abundance of Pasteurellaceae was decreased and that of Neisseriaceae was increased in patient group (p=0.008 and p=0.036, respectively). The relative abundance of Bacillales showed a negative correlation with disease stage (r=-0.638, p<0.001), while the relative abundance of Proteobacteria showed a positive correlation with the stage of disease (r=0.450, p=0.01). Conclusion: In our study, there were significant differences between the relative abundances of Pasteurellaceae and Neisseriaceae in the saliva of CRC patients. Additionally, the correlation between disease stage and abundance of Bacillales and Proteobacteria members was a novel finding, which needs validation in larger series. These findings might have implications for screening and prognostication of CRC. Keywords: Colorectal cancer, neisseriaceae, next-generation sequencing, oral microbiota, pasteurellaceae
Cite This Article
Guven D, Ergunay K, Brinkmann A, Alp A, Kittana F, Akyon Y, Yilmaz E, Karakoc D, Hamaloglu E, Yalcin S, Nitsche A, Hayran M, Dizdar O. A Snapshot of Oral Microbiota in Patients with Colorectal Cancer. EJMI. 2021; 5(2): 166-171
Corresponding Author: Deniz Can Guven